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KMID : 0604520070330040251
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Journal of the Society of Cosmetic Scientists of Korea
2007 Volume.33 No. 4 p.251 ~ p.262
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Antioxidative Activity, Component Analysis, and Anti-elastase Effect of Aspalathus linearis Extract
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Yang Hee-Jung
Won Bo-Ryoung
Lim Young-Jin
Yoon Sun-Kyeong
Ji Dong-Hwan
Choi Jee-Yeon
Han Seung-Joo
Lee Chung-Woo
Park Soo-Nam
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Abstract
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In this study, the antioxidative effects, inhibitory effects on elastase, and components of Aspalathus linearis extracts were investigated. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities () of extract/fractions of Aspalathus linearis were in the order: 50 % ethanol extract () < deglycosylated flavonoid aglycone fraction () < ethylacetate fraction (). Reactive oxygen species (ROS) scavenging activities () of some Aspalathus linearis extracts on ROS generated in system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activities were ethylacetate fraction () < deglycosylated flavonoid aglycone fraction () < 50 % ethanol extract (). 50 % Ethanol extract showed the most prominent scavenging activity. The protective effects of extract/fractions of Aspalathus linearis on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The Aspalathus linearis extracts suppressed photohemolysis in a concentration dependent manner, particularly 50 % ethanol extract exhibited the most prominent celluar protective effect (, 272.00 min at ). Aglycone fractions obtained from the deglycosylation reaction of ethylacetate fraction among the Aspalathus linearis extracts, showed 3 bands in TLC and 3 peaks in HPLC experiments (360 nm). Three components were identified as luteolin (composition ratio, 18.24 %), quercetin (58.79), and kaempferol (22.97). TLC chromatogram of ethylacetate fraction of Aspalathus linearis extract revealed 7 bands and HPLC chromatogram showed 9 peaks, which were identified as isoorientin (composition ratio, 14.71 %), orientin (28.84 %), vitexin (5.63 %), rutin and isovitexin (12.73 %), hyperoside (9.24 %), isoquercitrin (5.40 %), luteolin (1.48 %), quercetin (17.61 %) and kaempferol (4.59 %) in the order of elution time. The inhibitory effect of aglycone fraction on elastase () was very high. These results indicate that extract/fractions of Aspalathus linearis can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging and other ROS, and protect cellular membranes against ROS. And component analysis of Aspalathus linearis extract and inhibitory activity on elastase of the aglycone fraction could be applicable to new functional cosmetics for smoothing wrinkles.
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KEYWORD
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Aspalathus linearis, flavonoid, antioxidative activity, elastase, cosmetics
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